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mouse on mouse mom igg blocking reagent  (Vector Laboratories)


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    Structured Review

    Vector Laboratories mouse on mouse mom igg blocking reagent
    Mouse On Mouse Mom Igg Blocking Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mom+blocking+reagent/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Blocking+Reagent/pm41865375-312-7-14
    Average 96 stars, based on 1538 article reviews
    mouse on mouse mom igg blocking reagent - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Respiratory viral infections awaken metastatic breast cancer cells in lungs.
    Article Snippet: .. Sections were blocked for 1 h at room temperature with blocking solution (Abcam, AB64226) containing MOM blocking reagent (Vector Laboratories, MKB2213-1), incubated with primary antibodies (Supplementary Information Table 1) at 4 °C overnight in antibody diluent (Abcam, 64211), then washed 3 times for 30 min each in 0.1% triton-X 100 in PBS. .. For IHC samples, sections were incubated in ImmPRESS HRP goat anti-rabbit or rat IgG polymer detection kit (Vector Laboratories, MP-7451/MP7404) and ImmPACT DAB substrate, peroxidase HRP (Vector Laboratories, SK4105) according to the manufacturer’s instructions.

    Article Title: Tumor suppressors in Sox2-mediated lung cancers promote distinct cell-intrinsic and immunologic remodeling
    Article Snippet: For CK-5 staining, slides were blocked with 10% goat serum for 1–2 hours at room temperature before incubation with primary rabbit polyclonal anti–CK-5 antibody (1:500 dilution, Abcam ab52635) in 3% goat serum overnight. .. Washed slides underwent secondary antibody treatment with biotinylated anti-rabbit IgG (Biocare Medical GR608H) for 30 minutes. p63 slides were blocked with avidin and biotin before blocking with the MOM blocking reagent (Vector Lab BMK-2202) for mouse-on-mouse antibodies. p63 slides were incubated with primary mouse antibodies in MOM diluent (p63 Biocare Medical CM163A 1:100). .. All slides were then incubated with avidin-biotin complexes for 30 minutes and washed before being developed in DAB solution (Impact DAB from Vector Lab SK-4105) and counterstained in hematoxylin (Sigma-Aldrich GHS316).

    Article Title: Tumor suppressors in Sox2-mediated lung cancers promote distinct cell intrinsic and immunologic remodeling.
    Article Snippet: For CK-5 staining, slides were blocked with 10% goat serum for 1-2 hours at room temperature before incubation with primary rabbit polyclonal anti-CK-5 antibody (1:500 dilution, Abcam Cat#ab52635) in 3% goat serum overnight. .. Washed slides underwent secondary antibody treatment with biotinylated anti-rabbit IgG (Biocare Medical cat#GR608H) for 30 minutes. p63 slides were blocked with avidin and biotin before blocking with the MOM blocking reagent (Vector Lab CatBMK-2202) for mouse-on-mouse antibodies. p63 slides were incubated with primary mouse antibodies in MOM diluent (p63 Biocare Medical cat#CM163A 1:100). .. All slides were then incubated with avidin-biotin complexes for 30 minutes and washed before being developed in DAB solution (Impact DAB from Vector lab#SK4105) and counterstained in Hematoxylin (Sigma-Aldrich #GHS316).

    Article Title: Respiratory viral infections awaken metastatic breast cancer cells in lungs
    Article Snippet: .. Sections were blocked for 1 h at room temperature with blocking solution (Abcam, AB64226) containing MOM blocking reagent (Vector Laboratories, MKB2213-1), incubated with primary antibodies (Supplementary Information Table ) at 4 °C overnight in antibody diluent (Abcam, 64211), then washed 3 times for 30 min each in 0.1% triton-X 100 in PBS. .. For IHC samples, sections were incubated in ImmPRESS HRP goat anti-rabbit or rat IgG polymer detection kit (Vector Laboratories, MP-7451/MP7404) and ImmPACT DAB substrate, peroxidase HRP (Vector Laboratories, SK4105) according to the manufacturer’s instructions.

    Article Title: Specific 3 -O- sulfated heparan sulfate domains regulate salivary gland basement membrane metabolism and epithelial differentiation
    Article Snippet: .. SMGs were blocked for 1 h at room temperature with 10% heat-inactivated donkey serum (Jackson ImmunoResearch Laboratories, Westgrove, PA, USA), 1% BSA (Sigma, St Louis, MO, USA) and MOM blocking reagent (Vector Laboratories, Burlingame, CA, USA). .. Then incubated overnight at 4 °C with the appropriate primary antibody, then washed with PBST and incubated with the respective dye-conjugated secondary antibodies (all from Jackson ImmunoResearch Laboratories, Westgrove, PA, USA) and DAPI stain (EMD Millipore Corp, Billerica, MA, USA) for 1 h at room temperature.

    Article Title: Impaired cAMP–PKA–CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexia
    Article Snippet: .. Fresh slices were then incubated for 1 h at room temperature in MOM blocking reagent (MKB-2213-1; Vector Laboratories), washed in PBS and incubated overnight at 4 °C with the following primary antibodies: mouse anti-MyHC Type I (Myh7) clone BA-D5, mouse anti-MyHC Type IIa (Myh2) clone SC-71, and mouse anti-MyHC Type IIb (Myh4) clone BF-F3, all at 1:100 dilution (Developmental Studies Hybridoma Bank), and rabbit anti-laminin (Z0097; DAKO) at 1:200 dilution. .. Both C26 and LLC tumour bulk were minced in ~5-mm 3 parts and plated in serum-free DMEM supplemented with penicillin–streptomycin solution (Gibco; 15140-122).


    Incubation:

    Article Title: Respiratory viral infections awaken metastatic breast cancer cells in lungs.
    Article Snippet: .. Sections were blocked for 1 h at room temperature with blocking solution (Abcam, AB64226) containing MOM blocking reagent (Vector Laboratories, MKB2213-1), incubated with primary antibodies (Supplementary Information Table 1) at 4 °C overnight in antibody diluent (Abcam, 64211), then washed 3 times for 30 min each in 0.1% triton-X 100 in PBS. .. For IHC samples, sections were incubated in ImmPRESS HRP goat anti-rabbit or rat IgG polymer detection kit (Vector Laboratories, MP-7451/MP7404) and ImmPACT DAB substrate, peroxidase HRP (Vector Laboratories, SK4105) according to the manufacturer’s instructions.

    Article Title: Tumor suppressors in Sox2-mediated lung cancers promote distinct cell-intrinsic and immunologic remodeling
    Article Snippet: For CK-5 staining, slides were blocked with 10% goat serum for 1–2 hours at room temperature before incubation with primary rabbit polyclonal anti–CK-5 antibody (1:500 dilution, Abcam ab52635) in 3% goat serum overnight. .. Washed slides underwent secondary antibody treatment with biotinylated anti-rabbit IgG (Biocare Medical GR608H) for 30 minutes. p63 slides were blocked with avidin and biotin before blocking with the MOM blocking reagent (Vector Lab BMK-2202) for mouse-on-mouse antibodies. p63 slides were incubated with primary mouse antibodies in MOM diluent (p63 Biocare Medical CM163A 1:100). .. All slides were then incubated with avidin-biotin complexes for 30 minutes and washed before being developed in DAB solution (Impact DAB from Vector Lab SK-4105) and counterstained in hematoxylin (Sigma-Aldrich GHS316).

    Article Title: Tumor suppressors in Sox2-mediated lung cancers promote distinct cell intrinsic and immunologic remodeling.
    Article Snippet: For CK-5 staining, slides were blocked with 10% goat serum for 1-2 hours at room temperature before incubation with primary rabbit polyclonal anti-CK-5 antibody (1:500 dilution, Abcam Cat#ab52635) in 3% goat serum overnight. .. Washed slides underwent secondary antibody treatment with biotinylated anti-rabbit IgG (Biocare Medical cat#GR608H) for 30 minutes. p63 slides were blocked with avidin and biotin before blocking with the MOM blocking reagent (Vector Lab CatBMK-2202) for mouse-on-mouse antibodies. p63 slides were incubated with primary mouse antibodies in MOM diluent (p63 Biocare Medical cat#CM163A 1:100). .. All slides were then incubated with avidin-biotin complexes for 30 minutes and washed before being developed in DAB solution (Impact DAB from Vector lab#SK4105) and counterstained in Hematoxylin (Sigma-Aldrich #GHS316).

    Article Title: Respiratory viral infections awaken metastatic breast cancer cells in lungs
    Article Snippet: .. Sections were blocked for 1 h at room temperature with blocking solution (Abcam, AB64226) containing MOM blocking reagent (Vector Laboratories, MKB2213-1), incubated with primary antibodies (Supplementary Information Table ) at 4 °C overnight in antibody diluent (Abcam, 64211), then washed 3 times for 30 min each in 0.1% triton-X 100 in PBS. .. For IHC samples, sections were incubated in ImmPRESS HRP goat anti-rabbit or rat IgG polymer detection kit (Vector Laboratories, MP-7451/MP7404) and ImmPACT DAB substrate, peroxidase HRP (Vector Laboratories, SK4105) according to the manufacturer’s instructions.

    Article Title: Impaired cAMP–PKA–CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexia
    Article Snippet: .. Fresh slices were then incubated for 1 h at room temperature in MOM blocking reagent (MKB-2213-1; Vector Laboratories), washed in PBS and incubated overnight at 4 °C with the following primary antibodies: mouse anti-MyHC Type I (Myh7) clone BA-D5, mouse anti-MyHC Type IIa (Myh2) clone SC-71, and mouse anti-MyHC Type IIb (Myh4) clone BF-F3, all at 1:100 dilution (Developmental Studies Hybridoma Bank), and rabbit anti-laminin (Z0097; DAKO) at 1:200 dilution. .. Both C26 and LLC tumour bulk were minced in ~5-mm 3 parts and plated in serum-free DMEM supplemented with penicillin–streptomycin solution (Gibco; 15140-122).


    Avidin-Biotin Assay:

    Article Title: Tumor suppressors in Sox2-mediated lung cancers promote distinct cell-intrinsic and immunologic remodeling
    Article Snippet: For CK-5 staining, slides were blocked with 10% goat serum for 1–2 hours at room temperature before incubation with primary rabbit polyclonal anti–CK-5 antibody (1:500 dilution, Abcam ab52635) in 3% goat serum overnight. .. Washed slides underwent secondary antibody treatment with biotinylated anti-rabbit IgG (Biocare Medical GR608H) for 30 minutes. p63 slides were blocked with avidin and biotin before blocking with the MOM blocking reagent (Vector Lab BMK-2202) for mouse-on-mouse antibodies. p63 slides were incubated with primary mouse antibodies in MOM diluent (p63 Biocare Medical CM163A 1:100). .. All slides were then incubated with avidin-biotin complexes for 30 minutes and washed before being developed in DAB solution (Impact DAB from Vector Lab SK-4105) and counterstained in hematoxylin (Sigma-Aldrich GHS316).

    Article Title: Tumor suppressors in Sox2-mediated lung cancers promote distinct cell intrinsic and immunologic remodeling.
    Article Snippet: For CK-5 staining, slides were blocked with 10% goat serum for 1-2 hours at room temperature before incubation with primary rabbit polyclonal anti-CK-5 antibody (1:500 dilution, Abcam Cat#ab52635) in 3% goat serum overnight. .. Washed slides underwent secondary antibody treatment with biotinylated anti-rabbit IgG (Biocare Medical cat#GR608H) for 30 minutes. p63 slides were blocked with avidin and biotin before blocking with the MOM blocking reagent (Vector Lab CatBMK-2202) for mouse-on-mouse antibodies. p63 slides were incubated with primary mouse antibodies in MOM diluent (p63 Biocare Medical cat#CM163A 1:100). .. All slides were then incubated with avidin-biotin complexes for 30 minutes and washed before being developed in DAB solution (Impact DAB from Vector lab#SK4105) and counterstained in Hematoxylin (Sigma-Aldrich #GHS316).

    other:

    Article Title: Impaired cAMP-PKA-CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexia.
    Article Snippet: This plasmid was a gift from Feng Zhang (Addgene plasmid #62988; http://n2t.net/ addgene:62988; RRID: Addgene_62988)60.



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